Description
Quick Details
The MSLPCR03 Real Time fluorescence quantitative PCR System thermo cycler MSLPCR03 system adapts to almost any workflow requirement with flexible instrument control and data management.
>> Standard with 7’’ LCD touch screen, functions quickly accessible through simple, one-touch commands
>> High-throughput up to 96 samples per run
>> Push-pull, electrical controlled elevating platform
>> Concealed lid with constant temperature and isopiestic pressure bring high accurate temperature control
>> Quick-start set-up so you can begin a run immediately
>> Unique power-off protection function can save all the setted configurations for sudden power outages, and allow the experiment continues when power supply is restored.
>> System can be installed in three distinct configurations, providing unmatched flexibility and convenience.
1. Co-located configuration (PC control)
2. Standalone (PC-free) configuration (Data can be export by USB and analysis on PC)
3. Networked configuration (PC software automatically detect all systems in LAN and allow to remote monitoring one of experimental progress and downloading of the completed run file to the PC at your desk)
Packaging & Delivery
| Packaging detail:Standard export package Delivery detail:within 7-10 workdays after receipt of payment |
Specifications
MSLPCR03 Real Time fluorescence quantitative PCR System thermo cycler
The MSLPCR03 system adapts to almost any workflow requirement with flexible instrument control and data management.
>> Standard with 7’’ LCD touch screen, functions quickly accessible through simple, one-touch commands
>> High-throughput up to 96 samples per run
>> Push-pull, electrical controlled elevating platform
>> Concealed lid with constant temperature and isopiestic pressure bring high accurate temperature control
>> Quick-start set-up so you can begin a run immediately
>> Unique power-off protection function can save all the setted configurations for sudden power outages, and allow the experiment continues when power supply is restored.
>> System can be installed in three distinct configurations, providing unmatched flexibility and convenience.
1. Co-located configuration (PC control)
2. Standalone (PC-free) configuration (Data can be export by USB and analysis on PC)
3. Networked configuration (PC software automatically detect all systems in LAN and allow to remote monitoring one of experimental progress and downloading of the completed run file to the PC at your desk)
MSLPCR03 Real Time fluorescence quantitative PCR System
MSLPCR03 Real Time fluorescence quantitative PCR System thermo cycler
Fast and uniform heating and heat sinking block
>> Concealed lid with constant temperature and isopiestic pressure
>> Electrical controlled elevating & automatic locked platform
>>Thermo block is based on improved heat pipe technology, leading to extraordinary Iintra-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility.
>> Highly efficient PID&DSP control algorithm ensures faster respond and stronger interference resistance, fast and uniform heating and heat sinking
High sensitive and precise optical system
>> High-sensitivity and wide dynamic range of fluorescence detection system, sample dynamic range could be from 101 to 1010 copies
>> Fast and precise mechanical scanning structure ensures the illumination uniformity of 96 wells, Eliminate edge effects, no need for ROX calibration
>> Fluorescence excitation by a high-intensity and long-life LED, Bulb Luminance will not attenuation over time, free maintenance optical system through the entire life.
>> Professional designed fluorescence filters realize the highly fluorescence transmittance and meanwhile minimize the fluorescence channel crosstalk.
MSLPCR03 Real Time fluorescence quantitative PCR System
MSLPCR03 Real Time fluorescence quantitative PCR System thermo cycler Easy-to–use Software
The MSLPCR03 Software offers an intuitive experience for protocols programming, plate layouts setting and experimental results analysising. The Software includes an unlimited license for multiple users. The available software modules include Absolute quantification, Melting curve analysis, Relative quantification and High Resolution Melting (HRM). All the excellent features make the instrument to your ideal choice for qPCR.
>> Friendly interface and clearly functional modules design, first-time users can easily start a new experiment.
>> All experiment data and setting options are shown conveniently on a single interface. User modifications to analysis parameters can be seen instantly
>> Powerful analytical capabilities, optional automatic and manual threshold setting bring reliable date you can work on.
>> Experimental date can be export in .csv, .excel, .txt format, with this capability, the MSLPCR03 can interface with your laboratory information system directly.
>> Unique designed universal report can be customized according to the requirements, report review and print function help clinical documentation as needed.
This real-time PCR run was done using 5 ng of hepatitis B virus DNA in each of the 96 block positions. (A) A 135 bp amplicon of the hepatitis B virus gene was amplified using SYBR Green I detection. (B) The reaction product was also subjected to melting curve analysis. In summary, low variation Cq values (Cq range = 0.15and SD = 0.040) and overlapping melt curves of the amplicon (Tm range of 0.5°C and SD = 0.065) in each of the 96 positions demonstrated temperature homogeneity and equal treatment of all samples – independent of block position.
Which real-time or digital PCR system is right for you?
To understand why traditional PCR is limited, it is important to understand what happens during the PCR reaction. A basic PCR run can be divided into three phases:
Index period
Accumulated product exactly doubles with each cycle (assuming 100% reaction efficiency). The reaction is highly specific and precise. Exponential amplification occurs because all reagents are freshly available and the kinetics of the reaction drive the reaction in favor of amplicon doubling.
Linear phase (high variability)
As the reaction continues, some reagents are consumed by amplification. The reaction starts to slow down, and the PCR product no longer doubles with each cycle.
Plateau (endpoint: gel detection using conventional methods)
The reaction stops, no more product is produced, and if left long enough, the PCR product will start to degrade. Because each sample has different reaction kinetics, each tube or reaction will plateau at a different time point. These differences can be seen during the plateau. During the plateau period, conventional PCR performs the measurement, also known as endpoint detection.
Variation in plateau measurements with conventional PCR
Three replicate samples had the same amount of DNA at the start of the reaction and different amounts of PCR product during the plateau phase of the reaction (due to changes in reaction kinetics). Therefore, it will be more accurate to make measurements during the exponential period, where replicate samples expand exponentially.
Real-time PCR measures during exponential phase for more accurate quantitation
Real-time PCR focuses on the exponential phase because it provides more precise data for quantification. During the exponential period, the real-time PCR instrument calculates two values. The threshold line is the level of detection at which the fluorescence intensity of the reaction exceeds background. The PCR cycle at which the sample reaches this level is called the cycle threshold (Ct). Ct values are used for downstream quantification or presence/absence detection. By comparing the Ct values of a sample of unknown concentration with a series of standards, the amount of template DNA in an unknown reaction can be accurately determined.
Digital PCR counts individual molecules for absolute quantification
Digital PCR works by splitting the sample into many individual real-time PCR reactions; some of these reactions contain the molecule of interest (positive) and others do not (negative). After PCR analysis, a fraction of the negative results are used to generate absolute results for the exact amount of target molecule in the sample without the need for reference standards or endogenous controls.
MedsinglongPCR system, from kits, instruments, enzymes, master mixes, reagents, optical cartridges, plastic accessories, spare parts to software etc. Medsinglong real-time quantitative PCR system is a fully integrated quantitative PCR amplification, detection and data analysis system. Medsinglong increases productivity with its unique modular and flexible design, intuitive touch screen interface, advanced and easy-to-use reporting system, and monitors more than 120 attributes through built-in online diagnostic system, so that when analytical problems or instrument problems arise find out the reason.
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