Buy Real-Time PCR Detection System EKONPCR05 | real time pcr machine

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Buy Real-Time PCR Detection System EKONPCR05 | real time pcr machine

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Real-Time PCR Detection System EKONPCR05:

EKONPCR05is Bioer latest realtime PCR detection system. EKONPCR05has a faster heating and cooling rate,better temperature control accuracy,temperature uniformity and the machine stability withthe specially-madePeltier byFerrotec,advanced optical fiber technology.the new wide voltage power supply.its unique block radiating patent technology and the bottom detection patent mode.It can be used with Windows Tablet PC.This new touch screen operation will bring you the ultimateexperience ofhuman-computer interaction.The new and innovative LineGene K Plus Real-time PCRdetection system can adapt to different levels of customer requirements，which can fully meets various requirements of scientific research and clinical applications.

Buy Real-Time PCR Detection System EKONPCR05 | real time pcr machine Technical Parameters:

Buy Real-Time PCR Detection System EKONPCR05 | real time pcr machine

Which real-time or digital PCR system is right for you?

To understand why traditional PCR is limited, it is important to understand what happens during the PCR reaction. A basic PCR run can be divided into three phases:

Index period

Accumulated product exactly doubles with each cycle (assuming 100% reaction efficiency). The reaction is highly specific and precise. Exponential amplification occurs because all reagents are freshly available and the kinetics of the reaction drive the reaction in favor of amplicon doubling.

Linear phase (high variability) As the reaction continues, some reagents are consumed by amplification. The reaction starts to slow down, and the PCR product no longer doubles with each cycle.

Plateau (endpoint: gel detection using conventional methods) The reaction stops, no more product is produced, and if left long enough, the PCR product will start to degrade. Because each sample has different reaction kinetics, each tube or reaction will plateau at a different time point. These differences can be seen during the plateau. During the plateau period, conventional PCR performs the measurement, also known as endpoint detection.

Variation in plateau measurements with conventional PCR Three replicate samples had the same amount of DNA at the start of the reaction and different amounts of PCR product during the plateau phase of the reaction (due to changes in reaction kinetics). Therefore, it will be more accurate to make measurements during the exponential period, where replicate samples expand exponentially.

Real-time PCR measures during exponential phase for more accurate quantitation Real-time PCR focuses on the exponential phase because it provides more precise data for quantification. During the exponential period, the real-time PCR instrument calculates two values. The threshold line is the level of detection at which the fluorescence intensity of the reaction exceeds background. The PCR cycle at which the sample reaches this level is called the cycle threshold (Ct). Ct values are used for downstream quantification or presence/absence detection. By comparing the Ct values of a sample of unknown concentration with a series of standards, the amount of template DNA in an unknown reaction can be accurately determined.

Digital PCR counts individual molecules for absolute quantification Digital PCR works by splitting the sample into many individual real-time PCR reactions; some of these reactions contain the molecule of interest (positive) and others do not (negative). After PCR analysis, a fraction of the negative results are used to generate absolute results for the exact amount of target molecule in the sample without the need for reference standards or endogenous controls.