EKONPCR01 Thermal Cycler for pcr testing machines

Quick Details

EKONPCR01 Thermal Cycler for pcr testing machines Easy to use

>> EKONPCR01 is configured with 7’’ LCD touch screen, functions quickly accessible through simple, one-touch commands.

>> Compact design with very small footprint, very easy to be easily moved.

>> Operating system based on Android, specialized user training is not required, friendly interface make it simple even for first time users.

>> The graphic programming is quick, easy and intuitive.

>> Unlimited user accounts can be created, unlimited protocols for each account.

>> Personal account can be protected with a password.

>> Quick-start set-up so you can begin a run immediately on desktop.

Specifications

EKONPCR01 Thermal Cycler for pcr testing machines

EKONPCR01 Thermal Cycler for pcr testing machines Easy to use

>> EKONPCR01 is configured with 7’’ LCD touch screen, functions quickly accessible through simple, one-touch commands.

>> Compact design with very small footprint, very easy to be easily moved.

>> Operating system based on Android, specialized user training is not required, friendly interface make it simple even for first time users.

>> The graphic programming is quick, easy and intuitive.

>> Unlimited user accounts can be created, unlimited protocols for each account.

>> Personal account can be protected with a password.

>> Quick-start set-up so you can begin a run immediately on desktop.

EKONPCR01 Thermal Cycler for pcr testing machines

today’s scientist

EKONPCR01 - The New thermal cycler PCR from Tianlong, is quite easy to operate with the combination of a 7’’ LCD touch screen and an Android based operating system. By taking advantages of its excellent thermo block, EKONPCR01 brings you homogeneous and accurate temperatures, rapid and precisely controlled ramp rates and fast and reproducible PCR cycles. The programming of EKONPCR01 is also remarkably simple and intuitive. Thanks to all these advantages, EKONPCR01 is your ideal equipment for PCR.

Reproducibility

>> EKONPCR01 block features remarkable gradient technology to ensure that ramp rates are identical in both gradient and normal mode.

>> Optimized perform assay crosses a maximal 20℃range via 12 gradient temperatures.

Reliability

>>Specially designed lid reduce the evaporation during PCR and helps to accommodate a wide variety of PCR consumables.

>> The solid handle allows one hand operation of the instrument.

>> Unique power-off protection function can save all the set configurations after power off, and allow the experiment continues when power on.

EKONPCR01 Thermal Cycler for pcr testing machines

Flexibility

>> Protocols can be easily shared between different EKONPCR01s via USB flash disk, personal protocols can also be saved in your USB flash disk to quickly setup your own experiment on any EKONPCR01s.

EKONPCR01 Thermal Cycler for pcr testing machines

EKONPCR01 Thermal Cycler for pcr testing machines Specification:

Which real-time or digital PCR system is right for you?

To understand why traditional PCR is limited, it is important to understand what happens during the PCR reaction. A basic PCR run can be divided into three phases:

Index period

Accumulated product exactly doubles with each cycle (assuming 100% reaction efficiency). The reaction is highly specific and precise. Exponential amplification occurs because all reagents are freshly available and the kinetics of the reaction drive the reaction in favor of amplicon doubling.

Linear phase (high variability) As the reaction continues, some reagents are consumed by amplification. The reaction starts to slow down, and the PCR product no longer doubles with each cycle.

Plateau (endpoint: gel detection using conventional methods) The reaction stops, no more product is produced, and if left long enough, the PCR product will start to degrade. Because each sample has different reaction kinetics, each tube or reaction will plateau at a different time point. These differences can be seen during the plateau. During the plateau period, conventional PCR performs the measurement, also known as endpoint detection.

Variation in plateau measurements with conventional PCR Three replicate samples had the same amount of DNA at the start of the reaction and different amounts of PCR product during the plateau phase of the reaction (due to changes in reaction kinetics). Therefore, it will be more accurate to make measurements during the exponential period, where replicate samples expand exponentially.

Real-time PCR measures during exponential phase for more accurate quantitation Real-time PCR focuses on the exponential phase because it provides more precise data for quantification. During the exponential period, the real-time PCR instrument calculates two values. The threshold line is the level of detection at which the fluorescence intensity of the reaction exceeds background. The PCR cycle at which the sample reaches this level is called the cycle threshold (Ct). Ct values are used for downstream quantification or presence/absence detection. By comparing the Ct values of a sample of unknown concentration with a series of standards, the amount of template DNA in an unknown reaction can be accurately determined.

Digital PCR counts individual molecules for absolute quantification Digital PCR works by splitting the sample into many individual real-time PCR reactions; some of these reactions contain the molecule of interest (positive) and others do not (negative). After PCR analysis, a fraction of the negative results are used to generate absolute results for the exact amount of target molecule in the sample without the need for reference standards or endogenous controls.