Fast Real-time Fluorescence PCR Analyzer EKONPCR46 thermal cycler pcr machine

Quick Details

Fast Real-time Fluorescence PCR Analyzer EKONPCR46 thermal cycler pcr machine

Specifications

Fast Real-time Fluorescence PCR Analyzer EKONPCR46

highly sensitivity high specificity high stability Detection of respiratory pathogens, intestinal pathogens, food safety

Fast Real-time Fluorescence PCR Analyzer EKONPCR46 thermal cycler pcr machine

Fast real-time fluorescence PCR analyzer upgrade on traditional fluorescent quantitative PCR instrument, which not only retains the advantage of high detection sensitivity, but also overcomes the disadvantage of time-consuming reaction.

In this way, one-round reaction time can be shortened from 1.5 hours to 20~ 30min, which greatly saves time.

Fast Real-time Fluorescence PCR Analyzer EKONPCR46 thermal cycler pcr machine

●Its Windows Touch-Screen Operating System edit and run programmes by itself Instead of connecting to the computer. It can directly connect to the printer to print the report.

●EKONPCR46 Ultrafast Real-time PCR System is available with 4- color fluorescence channels, the temperature-dependent fluorescenceoptical system can acquire all channels simultaneously.

●Highly sensitive to rival ABI Quantstudio 6. ●Super instrment performance, 8~12*C/s temperature change speed, 20~ 30min to complete 40 cycles.

●DNA/RNA detetion available, Integrate traditional PCR/RT-PCR functions, and supports one step RT-PCR Method. ●Mobile portable and innovative compact design, especially suitable for on-site test and POCT. ●10uL reaction system saves reagents and samples.

1Sample type Pharynx swab, nasal swab, anal swab, oral swab, culture, bacterial culture suspension, serum, plasma, vomit, diarrhea, animal tissue, plant tissue, etc.

2.Multiplex Test Adopting 8 hole chip,8 samples and muti-target gene can be detected simnultaneously. 3.Simple and rapid The ultra-fast temperature rise and fall rate of 8~12C/s can shorten the PCR experiment from 1.5 hours to only 20~ 30 minutes. 4.Micro-reaction system Only 10μL reaction system is needed,reducing reagent cost.

5.High sensitivity Adopting microfluidic technology, each reaction pore is relatively independent and has no cross interference. As a result, the detection system has high sensitivity and can achieve the same sensitivity as ABI Q6.

6.Portable and durable Fast real-time fluorescence PCR analyzer weighs 8kg, which is so light and compact that can be carried to site and field in suitcases. Moreover, it can also be equipped with vehicle charging or storage battery and full power of it can run 10~12 rounds of PCR programs a day.

Which real-time or digital PCR system is right for you?

To understand why traditional PCR is limited, it is important to understand what happens during the PCR reaction. A basic PCR run can be divided into three phases:

Index period

Accumulated product exactly doubles with each cycle (assuming 100% reaction efficiency). The reaction is highly specific and precise. Exponential amplification occurs because all reagents are freshly available and the kinetics of the reaction drive the reaction in favor of amplicon doubling.

Linear phase (high variability) As the reaction continues, some reagents are consumed by amplification. The reaction starts to slow down, and the PCR product no longer doubles with each cycle.

Plateau (endpoint: gel detection using conventional methods) The reaction stops, no more product is produced, and if left long enough, the PCR product will start to degrade. Because each sample has different reaction kinetics, each tube or reaction will plateau at a different time point. These differences can be seen during the plateau. During the plateau period, conventional PCR performs the measurement, also known as endpoint detection.

Variation in plateau measurements with conventional PCR Three replicate samples had the same amount of DNA at the start of the reaction and different amounts of PCR product during the plateau phase of the reaction (due to changes in reaction kinetics). Therefore, it will be more accurate to make measurements during the exponential period, where replicate samples expand exponentially.

Real-time PCR measures during exponential phase for more accurate quantitation Real-time PCR focuses on the exponential phase because it provides more precise data for quantification. During the exponential period, the real-time PCR instrument calculates two values. The threshold line is the level of detection at which the fluorescence intensity of the reaction exceeds background. The PCR cycle at which the sample reaches this level is called the cycle threshold (Ct). Ct values are used for downstream quantification or presence/absence detection. By comparing the Ct values of a sample of unknown concentration with a series of standards, the amount of template DNA in an unknown reaction can be accurately determined.

Digital PCR counts individual molecules for absolute quantification Digital PCR works by splitting the sample into many individual real-time PCR reactions; some of these reactions contain the molecule of interest (positive) and others do not (negative). After PCR analysis, a fraction of the negative results are used to generate absolute results for the exact amount of target molecule in the sample without the need for reference standards or endogenous controls.

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