Gradient PCR Thermal Cyclers Real Time PCR System

Quick Details

The most advanced peltier-based semiconductor technology.

High-performance universal power supply.

Large 5.7-inch high-definition LCD display.

Graphical user interface in English and Chinese.

Gradient PCR Thermal Cyclers Real Time PCR System

Specifications

Gradient PCR Thermal Cyclers Real Time PCR System

Gradient PCR Thermal Cyclers Real Time PCR System Products used the most advanced semiconductor technology, with theimport of original power supply and other key core components, effectively improve the overall performance of the product to ensure products’ amplified speed, extend life, and protect the reliability of analysis results in harsh and complex environments Under the experiment.

Cheap Gradient PCR Thermal Cyclers features

Gradient PCR Thermal Cyclers Real Time PCR System

The most advanced peltier-based semiconductor technology.

High-performance universal power supply.

Large 5.7-inch high-definition LCD display.

Graphical user interface in English and Chinese.

Power-down data protection.

Metal shell, solid, practical, beautiful and generous.

Stepless adjustable hot lid.

Lid can be positioned at any angle.

High-sealing reaction zone, to ensure stable and reliable test.

MSL Gradient PCR Thermal Cyclers technical specifications

Capacity 96×0.2ml, 96×0.2ml+77×0.5ml 384well

Temperature Range 0～99.9℃

Heating Rate 4℃/s

Cooling Rate 4℃/s

Uniformity ≤±0.3℃

Accuracy ≤±0.2℃

Display Resolution 0.1℃

Temperature Control Block\Tube

Ramping Rate Adjustale 0.1～4℃

Gradient Uniformity ≤±0.3℃

Gradient Accuracy ≤±0.2℃

Gradient Temp. Range 30～99.9℃

Gradient Spread 1～30℃

Hot Lid Temperature 30～110℃

Hot Lid Height Adjustale Stepless Adjustable

Number of Programs 200

Max.No.of Segment 9

Max.No.of Step 9

Max.No.of Cycle 99

Time Increment/Decrement 1 Sec ～ 9 Min 59 Sec

Temp. Increment/Decrement 0.1～9.9℃

Pause Funcation Yes

Auto Data Protection Yes

Graphical Display Yes

Hold at 4℃ Forever

Language 中文/English

LCD 5.7inch，320×240 pels

Communication USB2.0, RS232

Dimensions 392mm×262mm×252mm (L×W×H)

Weight 10kg

Power Supply 85～264VAC, 50～60Hz, 600 W

Gradient PCR Thermal Cyclers Real Time PCR System

Which real-time or digital PCR system is right for you?

To understand why traditional PCR is limited, it is important to understand what happens during the PCR reaction. A basic PCR run can be divided into three phases:

Index period

Accumulated product exactly doubles with each cycle (assuming 100% reaction efficiency). The reaction is highly specific and precise. Exponential amplification occurs because all reagents are freshly available and the kinetics of the reaction drive the reaction in favor of amplicon doubling.

Linear phase (high variability) As the reaction continues, some reagents are consumed by amplification. The reaction starts to slow down, and the PCR product no longer doubles with each cycle.

Plateau (endpoint: gel detection using conventional methods) The reaction stops, no more product is produced, and if left long enough, the PCR product will start to degrade. Because each sample has different reaction kinetics, each tube or reaction will plateau at a different time point. These differences can be seen during the plateau. During the plateau period, conventional PCR performs the measurement, also known as endpoint detection.

Variation in plateau measurements with conventional PCR Three replicate samples had the same amount of DNA at the start of the reaction and different amounts of PCR product during the plateau phase of the reaction (due to changes in reaction kinetics). Therefore, it will be more accurate to make measurements during the exponential period, where replicate samples expand exponentially.

Real-time PCR measures during exponential phase for more accurate quantitation Real-time PCR focuses on the exponential phase because it provides more precise data for quantification. During the exponential period, the real-time PCR instrument calculates two values. The threshold line is the level of detection at which the fluorescence intensity of the reaction exceeds background. The PCR cycle at which the sample reaches this level is called the cycle threshold (Ct). Ct values are used for downstream quantification or presence/absence detection. By comparing the Ct values of a sample of unknown concentration with a series of standards, the amount of template DNA in an unknown reaction can be accurately determined.

Digital PCR counts individual molecules for absolute quantification Digital PCR works by splitting the sample into many individual real-time PCR reactions; some of these reactions contain the molecule of interest (positive) and others do not (negative). After PCR analysis, a fraction of the negative results are used to generate absolute results for the exact amount of target molecule in the sample without the need for reference standards or endogenous controls.